Directed Evolution: Methods and protocols
Andrew Currin, Neil Swainston
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
درجه (قاطیغوری(:
کال:
2022
خپرندویه اداره:
Humana
ژبه:
english
صفحه:
280
ISBN 10:
1071621513
ISBN 13:
9781071621516
لړ (سلسله):
Springer Protocols
فایل:
PDF, 7.01 MB
IPFS:
,
english, 2022